Motility Assays for Motor Proteins (Methods in Cell Biology, Vol 39)

  • 269 Pages
  • 0.34 MB
  • English
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Cellular biology, Developmental biology, Neurosciences, Proteins, Scientific equipment & techniques, laboratory equipment, Molecular Biology, Science, Science/Mathem
The Physical Object
ID Numbers
Open LibraryOL9499922M
ISBN 100126289204
ISBN 139780126289206

Search in this book series. Motility Assays for Motor Proteins. Edited by Jonathan M. Scholey. Vol Pages ii-xv, () Download full volume. Previous volume. Next volume. Actions Motility Assays for Motor Proteins book selected chapters.

Select all / Deselect all. Download PDFs Export citations. Motility assays allow the visualization of individual motor proteins moving in vitro, and the movement of such motors as they drive microtubule-based motility events in living cells. Myosin-specific adaptations of the motility assay / James R.

Sellers, et al. --Motility of myosin I on planar lipid surfaces / henry G. Zot and Thomas D.

Description Motility Assays for Motor Proteins (Methods in Cell Biology, Vol 39) FB2

Pollard --Microtubule and exoneme gliding assays for force production by microtubule motor proteins / Bryce M.

Paschal and Richard B. Vallee --Analyzing microtubule motors in real time / S.A. Purchase Motility Assays for Motor Proteins, Volume 39 - 1st Edition.

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ISBNBook Edition: 1. Genre/Form: Electronic books: Additional Physical Format: Print version: Motility assays for motor proteins. San Diego: Academic Press, © (OCoLC) Just three families of motor proteins — myosin, kinesin, and dynein—power most eukaryotic cellular movements (Fig.

and Table ).During evolution, myosin, kinesin, and Ras family guanosine triphosphatases (GTPases) appear to have shared a common ancestor (Fig. ), whereas dynein is a member of the AAA adenosine triphosphatase (ATPase) family (Box ). Motility assays for motor proteins (methods in cell biology, vol.

39) edited by Jonathan M. Scholey Academic Press, £ pbk ISBN 0 12 / £ hbk ISBN 0 12 7Cited by: 4. Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms.

In this introductory lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). A Dynamical Model of Kinesin-Microtubule Motility Assays Article in Biophysical Journal 80(6) July with 25 Reads How we measure 'reads'.

Besides the motor proteins above, there are many more types of proteins capable of generating forces and torque in the cell. Many of these molecular motors are ubiquitous in both prokaryotic and eukaryotic cells, although some, such as those involved with cytoskeletal elements or chromatin, are unique to motor protein prestin, expressed in mammalian.

Motility Assays for Motor Proteins book Murray J.W., Wolkoff A.W. () In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting. In: Sperry A.O.

(eds) Molecular Motors. Methods in Cited by: 6. Plant Cell Biology, volume in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics.

The single-molecule motility assay can be performed on bundles of actin [50, 51] or with actin-containing regulatory proteins such as tropomyosin. Actin filament meshworks can be created in vitro, and it is seen that myosin 5a can readily make abrupt turns and switch from one filament to another or can “step over” an intersecting filament Cited by: 3.

Protein interactions of bacterial motility proteins. Decades of research have identified many components of bacterial flagella and their motors (Supplementary Table S1).We have used most of these motility proteins in two-hybrid screens in both T.

pallidum and C. jejuni. All known T. pallidum motility proteins were tested as fusions to the Gal4-DNA binding domain.

Mechanics of Motor Proteins and the Cytoskeleton - Sinauer Associates, Inc. of Motor Proteins and the Cytoskeleton was inspired by Dr.

Howard's teaching of a course on Cell Motility at the University of Washington. Back to top Reviews and Commentary "The cytoskeleton is an area of intense research and we are in danger of drowning in a sea of facts.

Motor proteins have two parts: a motor domain that utilizes adenosine triphosphate (ATP) hydrolysis to produce movements and a tail that allows the motors to self-associate or to bind particular cargo.

Within the three families, the tails are more diverse than the motor domains, allowing for specialized functions of each motor isoform. In Vitro Motility Assays—Skeletal muscle myosin was prepared from back muscle of male New Zealand White rabbits according to Margossian and Lowey and stored at –20 °C in 50% glycerol for up to 4 meromyosin (HMM) was prepared by mild chymotryptic digestion ( μg/ml) of myosin as described and used within 1 week.

motor protein: n. A protein, such as myosin, that converts chemical energy into mechanical energy, generating force that powers cell movement and the transport of substances and organelles within cells.

Please be advised that we experienced an unexpected issue that occurred on Saturday and Sunday January 20th and 21st that caused the site to be down for an extended period of time and affected the ability of users to access content on Wiley Online Library.

The movement of pollen tube organelles relies on cytoskeletal elements. Although the movement of organelles along actin filaments in the pollen tube has been studied widely and is becoming progressively clear, it remains unclear what role microtubules play.

Many uncertainties about the role of microtubules in the active transport of pollen tube organelles Cited by: About. Cell Movements: From Molecules to Motility (second edition) by Dennis Bray (google books entry) is a book I read because I thought I might do a PhD related to cell movement - more specifically, creating a computer modelling framework for summary is mostly a compilation of concepts from each chapter, plus a few extra interesting facts.

Sequences of 11 core motor proteins (C-ring proteins FliG, FliM, and FliN, T3SS proteins FlhB, FliI, FliP, and FliR, proximal rod proteins FlgB, Cited by: In in vitro motility assays, full-length Cut7 moved toward minus-end of microtubules, but the N-terminal half of Cut7 moved toward the opposite direction.

Furthermore, additional truncated constructs lacking the N-terminal or C-terminal regions, but still contained the motor domain, did not switch the motile direction. We investigate a microscopic model of motility assays in which actin filaments move over a layer of myosin heads attached to a glass plate.

The actin filaments are regarded as unstretchable semi-flexible polymer chains, and we use a standard method Cited by: 1. Learn cells biology motor proteins with free interactive flashcards. Choose from different sets of cells biology motor proteins flashcards on Quizlet.

By the end of the s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified.

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At the time, these two motors seemed almost sufficient to explain directional motility events on. Cell Movements vividly describes how complex movements can arise from the properties and behaviors of biological molecules. This second edition is updated throughout with recent advances in the field and has a completely revised and redrawn artwork program.

The text is suitable for advanced undergraduates as well as for professionals wishing for an overview of. these proteins and to increase the stability of these proteins. This chapter focuses on the integration of kinesin molecular motors and their microtubule tracks into microdevices for bioanalytical applications.

A number of insightful reviews have been written on the molecular mechanism of motor proteins in general,14,15 and kinesin motors in. In vitro motility assays enabled the identification of important factors that are essential for the regulation of intracellular transport including motor proteins and their membrane receptors, the formation of hetero-motor complexes and transported cargoes.

12 Structures of Motor Proteins Crossbridges and the Domain Organization of Motor Proteins Motor Families High-Resolution Structures Docking of Motors to Their Filaments Summary 13 Speeds of Motors The Speeds of Motors in Vivo Rowers and Porters In Vitro Motility Assays Processive and Nonprocessive Motors.

Kinesin-5 is a molecular motor protein that is essential in mitosis. Kinesin-5 proteins are members of kinesin superfamily, which are nanomotors that move along microtubule tracks in the cell. Named from studies in the early days of discovery, it is also known as kinesin family mem encoded by the KIF11 gene, or as BimC, Eg5 or N-2, based on the founding members of this Aliases: KIF11, EG5, HKSP, KNSL1, MCLMR.

Motility Determinants in WASP Family Proteins. This is the final version - click for previous version. on the beads was determined by Western blotting with an anti-FLAG antibody by using known amounts of the FLAG-tagged proteins as a standard.

For motility assays, beads were coated with a final concentration of 10–20 pmol of protein bound.For each of the protein assays presented here, 14 proteins were assayed using the standard test tube protocol.

The net (blank corrected) average absorbance for each protein was calculated. The net absorbance for each protein is expressed as a ratio to the net absorbance for BSA (e.g., a ratio of means that the protein produces 80% of the.